Coding
AtPHT1;5

Part:BBa_K4213002:Design

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-08-26)


PHT1;5 from A. thaliana optimized


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 868
    Illegal PstI site found at 497
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 868
    Illegal PstI site found at 497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 868
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 868
    Illegal PstI site found at 497
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 868
    Illegal PstI site found at 497
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-Subpart of Kozak frame added -Codon optimization for N.benthamiana



Source

Arabidopsis thaliana

References